KINETICfinder^®

There is a very high correlation between the K_d, k_on and k_off values obtained from our assays and the IC₅₀ and kinetics values obtained from activity assays and biophysical methods such as Surface Plasmon Resonance (SPR). Moreover, our assays provide reliable results regardless of the magnitude of the value measured.

We are experts in kinetic profiling

Enzymlogic is a world leading analytical CRO, expert in generation and interpretation of kinetic data. We are proud to serve the smallest biotech innovators through to 10 of the world’s top 20 Pharma organizations, supporting drug hunters in their search for the most promising compounds in early discovery.

References

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All our assays go through a rigorous establishment and validation process prior to use and ongoing assay performance is closely monitored to ensure they meet the highest of standards. KINETICfinder^® provides precise and reproducible K_d, k_on and k_off readouts, regardless of the magnitude of the values, in just a single assay.

Figure 2. KINETICfinder® provides accurate and reproducible results with a wide dynamic range. K_d, k_on and k_off values of 140 inhibitors and 140 different kinases from two independent experiments are shown on scatter plots with the axes in logarithmic scale. Each point represents an inhibitor-kinase interaction (875 interactions total). The plots display the Pearson correlation coefficient (r) and p-value.

KINETICfinder^® is a flexible and highly sensitive platform designed for the identification and discrimination of a wide spectrum of compounds:

• Slow to rapid binders
• Weak to potent binders.

Our assays use low protein concentrations (from picomolar to low nanomolar), precise TR-FRET readouts and continuous measurements that afford a broad dynamic range:

• K_d: mM-pM (8 log)
• k_on: 10¹-6×10⁷ M^-1s^-1 (7 log)
• k_off: 10^-6-0.5 s^-1 (7 log)

The high and sustained stability of our assays guarantees superior quality results over time.

• Outstanding reproducibility between plates and days.
• Enables data to be interpreted with confidence.
• High Z-values ensure low false positive/negative rates.

Our assays ensure robust measurement over time

Our assays are highly reproducible between plates and days

Enzymlogic’s kinetic profiling assays are performed in solution, benefiting clients who have experienced limitations when using biosensing technologies like SPR, stemming from complications due to target immobilization on the sensor chip.

Enzymlogic’s kinetic profiling assays are performed in solution, benefiting clients who have experienced limitations when using biosensing technologies like SPR, stemming from complications due to target immobilization on the sensor chip.

In addition, Enzymlogic assays exhibit higher reproducibility vs SPR given that, in each run we:

• Measure multiple concentrations of each compound.
• Measure several compounds (up to 80).
• Use internal reference compounds.

Also, as chips have a finite lifetime, the use of different SPR chips when building a dataset is also a source of variability.

Talk to us about your kinetic profiling roadblocks. We love a challenge and have successfully supported clients where other approaches have not worked out.

Quality data delivered on schedule

We are laser focused on rapid and reliable data delivery, with a keen eye on timelines and budgets.

• Standard data turnaround for study requests is 10 business days from compound receipt.
• Expedited turnaround is available for regularly scheduled ongoing screening programs.

We offer flexible solutions

Together we will define the best way to collaborate through:

• Ready-to-use or user-defined assays.
• User-defined screening concentrations.
• Bespoke assay development.
• Flexible service models to support your discovery goals and maximize program success rates.
• Direct contract, sub-contract via your principal CRO service provider or using outsourcing services (including Scientist and Science Exchange).